Figure 2.
Cylindrical capillary versus flat grid for single-cell mounting in SXT
(A) Schematics of flat grid and glass capillary cell mounting for SXT. Depicted in yellow: SXT field of view is about 16 μm × 16 μm with 50-nm objective lens. X-ray progressive absorption (from yellow to white) limits depth of imaging and maximum rotation angle for flat specimens, resulting in a “missing wedge.”
(B) In a cylindrical sample holder (black arrows), the full cell volume of a single cell is imaged, seen in both axial (XY) and sagittal (XZ) view. The gray values show the different level of absorption of each type of organelle with a threshold set from 0.1 to 0.5 μm−1.
(C) The effect of limited ±65° rotation, there is “shadowing” with the sample holder and decrease in X-ray transmission in comparison with full-rotation tomography. Arrows show streak artifacts. (D) Calculated X-ray radiation dose for limited angle and full-rotation tomography in Gy. The colorbar is from 1.5 × 107 to 1.5 × 108 Gy. Scale bars are 1 μm.