Figure 5.

Quantitative analysis of changes in organelle morphology and composition at 6 and 24 hpi as compared with uninfected HEK293T-ACE2 cells

(A–D) Exemplarily 3D renderings of (A) mitochondria, (B) lipid bodies, (C) vesicles, and (D) apoptotic bodies, and their corresponding quantitative analysis of total volume (normalized to total cell volume) and soft X-ray absorption coefficient (LAC). Each organelle is color coded as follows: mitochondria - green, lipid bodies - yellow, vesicles - cyan, large apoptotic bodies - lavender. Statistical significance is reported for lipid droplet radius in mock cells compared with 24 hpi (∗∗∗∗p < 0.0001) and 6 hpi cells compared with 24 hpi (∗∗p = 0.0042). Similarly, we report a significance difference in the vesicle radius at 6 hpi compared with mock cells (∗∗∗p = 0.0006) and for the X-ray absorption of the vesicles in mock cells compared with 24 hpi (∗∗∗∗p < 0.001) and compared with 6 hpi (∗∗p < 0.0020) (Dunnett’s multiple comparison test). The statistical analysis for all organelles is performed based on Tukey’s multiple comparison test.