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. 2021 Jun 9;9(1):10.1128/spectrum.00145-21. doi: 10.1128/spectrum.00145-21

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Copyright © 2021 Hoffman et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 1 — Characterization of the expression of KSHV ORF20. (A) Diagram showing the genomic locus of ORF20, with surrounding gene ORF21, depicting the location of the introduced mutation. (B) ORF20 expression was assessed by Western blotting in both iSLK.WT and ORF20STOP cells whether in their latent phase (Lat) or lytic phase (Lyt). DHX9 served as a loading control. (C) Western blot showing the expression kinetics of ORF20 in iSLK.WT cells (top) and TREX-BCBL1 cells (bottom) after reactivation with doxycycline and sodium butyrate for the indicated times. ORF59 (early kinetic) and K8.1 (late kinetic) expression is shown as controls. Vinculin served as a loading control.