FIG 2.
Determination of ORF20 proximal interactome. (A) Schematic representation of the BioID assay, where ORF20 is fused to the biotin ligase BirA which will transfer a biotin to proteins coming in close proximity to ORF20. Expression of the BirA-ORF20 fusion is shown on the right. Vinculin serves as a loading control (B) iSLK.ORF20STOP cells were transfected with ORF20-BirA and subjected to BioID and mass spectrometry. The bar graph represents the top GO terms associated with ORF20 interacting partners, and the network, generated by Cytoscape, represents the ORF20 proximal interactome. Proteins annotated in the categories highlighted in the bar graph are shown in clusters.