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Lack of ORF20 reduces virion production. (A) Diagram depicting the supernatant transfer assay in iSLK cells. (B) Supernatant transfer assay was used as a proxy for virion production and performed as described in panel A. Infection of live HEK293T cells was monitored by contrast (top) and green fluorescent protein (GFP) imaging (bottom) on a fluorescence microscope. (C) Quantification of GFP-positive cells using ImageJ. Values represent four independent views of the infected cells.
