TABLE 2.
Sequences of oligonucleotide primers used in this study
| Primer name | Oligonucleotide sequence (5′–3′) (restriction enzyme)a |
|---|---|
| Psip1Ab1-L-F | AACTGCAGCAGCTATAACATATGATGGA (PstI) |
| Psip1Ab1-L-R | CGGGATCCAATTATCCCTCCATCATTC (BamHI) |
| Psip1Ab1-S-F | AACTGCAGCATCCGTTAAAGGAAGCATGAATTAGTAATG (PstI) |
| Psip1Ab1-F-R | CGGGATCCTTTAAAAGTGACCTTAAAAAAATCC (BamHI) |
| sip1Ab1-F | AACTGCAGCAGCTATAACATATGATGGA (PstI) |
| sip1Ab1-R | CGGGATCCTTAATTTCCACTTAAAATCTTTGTTTGAACAGG (BamHI) |
| 304-F | CTATGACCATGATTACGCCAAGCTTGC |
| 304-S | GGATGTGCTGCAAGGCGATTAAGTTGG |
| 304-18Z F | CGTAATCTTACGTCAGTAACTTCCACAGTA |
| 304-18Z R | CGCCAGGGTTTTCCCAGTCACGAC |
| abrB-a | TATCGATGCATGCCATGGTACCCGGGCATATGCCTGTGGCGTAATAT |
| abrB-b | CCTCAAATGGTTCGCTGTTTTAGATTCGTC ATTTTTCG |
| Km-F | CGAAAAATGACGAATCTAAAACAGCGAACCATTTGAGG |
| Km-R | GCTTATTTTG CTGTTTCGAT ATAAAATTCC TCGTAGGCGC |
| abrB-c | GCGCCTACGA GGAATTTTAT ATCGAAACAG CAAAATAAGC |
| abrB-d | TCGACGCGTC TGCAGAAGCT TCTAGAATTC TACGAGTTAT CATGAGCAC |
| AbrB-F | CGGATCCGATTATGAAATCTACTGGTATTG (BamHI) |
| AbrB-R | GCGTCGACTTTTGCTGTTTCGATATAATCT(SalI) |
| JDWΔAbrB-F | GCCATGAGCAAAGACTTTCTTCGGA |
| JDWΔAbrB-R | GGTAGAGTGCTCTGATGGAAGTTAT |
| sip1Ab15′race-R | CTTTAAATATCCAGAATCTAGCTAAAAACCATCC |
| Psip1-S-F | CATCCGTTAAAGGAAGCATGAATTA |
| Psip1-S-R | AATTATCCCTCCATCATTCTTTTTG |
| Psip1-F-F | CAGCTATAACATATGATGGAAGAGAAA |
| Psip1-F-R | TTTAAAAGTGACCTTAAAAAAATCC |
| M13F-FAM | GTAAAACGACGGCCAGT (5′ FAM labeled) |
| M13R-FAM | CAGGAAACAGCTATGAC (5 FAM labeled) |
a
FAM, 6-carboxyfluorescein. The restriction sites are underlined and bolded.