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Assay specificity determination. (A) DBS and serum collected prior to December 2019 were tested using three lots of beads for DBS (A, B, C) and one bead lot for serum. *, P < 0.005 (B) Serum specimens containing antibodies to respiratory viruses, including other human coronaviruses, were tested. Lower line is cutoff for indeterminate results; upper line is cutoff for reactive results. MFI, median fluorescence intensity; N, nucleocapsid; S1, spike subunit 1.
