TABLE 2.
NRPS adenylation domain specificity predictiona
| Module | Domain specificity predicted by method |
Corresponding amino acid | |||||||
|---|---|---|---|---|---|---|---|---|---|
| LSI based |
NRPSsp |
PKS/NRPS |
SEQL-NRPS |
||||||
| Pred | Score (%) | Pred | Score (HMMER bit) | Pred | Score (bits) | Pred | Prob | ||
| RthA | |||||||||
| 1 | Glu | 0.595 | Ser | 661.3 | Glu | 16.5 | Ser | 0.485 | Lipo-d-Cit |
| 2 | l-allo-Thr | ||||||||
| 3 | Arg | 0.528 | Ser | 568.5 | Ser | 16.9 | Ser | 0.474 | l-Dab |
| 4 | Asn | 0.934 | Asn | 486.6 | Asn | 16.5 | Asn | 0.615 | l-Asn-OH |
| 5 | Asn | 0.700 | Asn | 450.4 | Asn | 18.1 | Orn | 0.463 | l-Asn |
| RthB | |||||||||
| 1 | Thr | 0.955 | Thr | 651.9 | Thr | 19.2 | Thr | 0.469 | Thr |
a
The specificities of the A domains of the different modules in RthA and RthB were predicted using different software tools. Pred, predicted amino acid; Prob, probability.