FIG 5.

kdpA mRNA levels in C. crescentus. The expression was determined by RT-qPCR from the total RNA isolated from cultures growing in M2 medium, calculated as 2^-ΔΔCT. (A) Expression was measured either in the wild-type (wt) strain NA1000 or in the sigT strain using total RNA from cultures incubated for 2 h in sodium phosphate-based M2 medium (M2-K) with sufficient (1 mM KCl) or deficient (50 μM KCl) potassium levels. The expression was determined relative to the respective culture in the K⁺-sufficient condition. (B) Expression was measured either in the wild-type strain NA1000 (wt) or in the sigT strain using the total RNA from cultures incubated in M2 medium at 30°C (Cold -) and after 2 h at 10°C (Cold +). The expression was determined relative to the wt strain at 30°C. (C) Expression from cells grown in M2 medium at 30°C from the wild-type strain (wt) and in the rho mutant strain (NA1000 rho::Tn5) and rhlE mutant strain (NA1000 rhlE::Tn5) (Table S2). (D) The same as in part C, but cultures were grown at 10°C. The results shown are from 4 independent experiments using RNA from 2 biological replicas. The significance was calculated using Student’s t test (*P < 0.001).