Table 1. Sequences of primers for resistance genes PCR amplification.
Gene | Primersa | Primer sequences (5′-3′) | Expected amplicon size (bp) |
---|---|---|---|
TEM | F R |
ATAAAATTCTTGAAGACGAAA GACAGTTACCAATGCTTAATC |
1,080 |
SHV | F R |
TGGTTATGCGTTATATTCGCC GGTTAGCGTTGCCAGTGCT |
865 |
CTX-M-1 | F R |
AAAAATCACTGCGCCAGTTC AGCTTATTCATCGCCACGTT |
415 |
CTX-M-2 | F R |
CGACGCTACCCCTGCTATT CCAGCGTCAGATTTTTCAGG |
552 |
CTX-M-8 | F R |
TCGCGTTAAGCGGATGATGC AACCCACGATGTGGGTAGC |
666 |
CTX-M-9 | F R |
CAAAGAGAGTGCAACGGATG ATTGGAAAGCGTTCATCACC |
205 |
CTX-M-25 | F R |
GCACGATGACATTCGGG AACCCACGATGTGGGTAGC |
327 |
OXA-1 | F R |
CTGTTGTTTGGGTTTCGCAAG CTTGGCTTTTATGCTTGATG |
440 |
OXA-2 | F R |
CAGGCGCYGTTCGYGATGAGTT GCCYTCTATCCAGTAATCGCC |
233 |
OXA-10 | F | GTCTTTCRAGTACGGCATTA | 822 |
R | GATTTTCTTAGCGGCAACTTA | ||
VEB | F R |
GCGGTAATTTAACCAGA GCCTATGAGCCAGTGTTC |
961 |
GES | F R |
ATGCGCTTCATTCACGCAC CTATTTGTCCGTGCTCAGG |
846 |
PER | F R |
AGTCAGCGGCTTAGATA CGTATGAAAAGGACAATC |
978 |
Notes.
Primer, The ‘F’ meant the forward primer and the ‘R’ meant the reverse primer.