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. 2021 Jul 21;9(1):10.1128/spectrum.00305-21. doi: 10.1128/spectrum.00305-21

FIG 2.

FIG 2

The S. carnosus CA deletion mutant cannot grow in atmospheric air and is interchangeable with mpsABC from S. aureus. (A) The S. carnosus CA deletion mutant cannot grow in atmospheric air (0.04% CO2) but can be restored at 5% CO2 and can also be complemented with mpsABC from S. aureus. Clockwise from top: S. carnosus TM300 wild type (Sc), S. carnosus TM300 CA deletion mutant (ScΔcan), and ScΔcan complemented with plasmid pRB473 carrying mpsABC-Sa (Δcan mpsABC). Likewise, CA from S. carnosus can also complement S. aureus ΔmpsABC. (B) Clockwise from top: S. aureus HG001 ΔmpsABC (SaΔmpsABC), S. aureus HG001 ΔmpsABC complemented with plasmid pRB473 carrying CA from S. carnosus (SaΔmpsABC can), and S. aureus HG001 ΔmpsABC carrying empty plasmid pRB473 as a control (ΔmpsABC pRB473). Plates were incubated in atmospheric air (left) and 5% CO2 (right).