TABLE 3.
Occurrence of MpsAB, CA, and intercellular adhesion gene (ica)-encoded protein cluster in selected staphylococcal species
| Protein(s) and species | Presence of MpsAB, CA, or Ica proteina |
||||
|---|---|---|---|---|---|
| MpsAB | CA | IcaA | IcaB | IcaC | |
| Group i: MpsAB and Ica | |||||
| Staphylococcus argenteus BN75 | + | − | + | + | + |
| Staphylococcus aureus subsp. aureus MSHR1132 | + | − | + | + | + |
| Staphylococcus aureus subsp. aureus USA300_FPR3757 | + | − | + | + | + |
| Staphylococcus capitis AYP1020 | + | − | + | + | + |
| Staphylococcus epidermidis RP62A | + | − | + | + | + |
| Staphylococcus lugdunensis C_33 | + | − | + | + | + |
| Staphylococcus simiae NCTC 13838 | + | − | + | + | + |
| Staphylococcus xylosus SMQ121 | + | − | + | + | + |
| Staphylococcus saprophyticus 883 | + | − | ++ | ++ | ++b |
| Staphylococcus cohnii SNUDS-2 | + | − | + | − | + |
| Staphylococcus sciuri SNUSD-18 | (+) | + | ++ | − | ++ |
| Group ii: CA only | |||||
| Staphylococcus agnetis 908 | − | + | − | − | − |
| Staphylococcus carnosus TM300 | − | + | − | − | − |
| Staphylococcus felis ATCC 49168 | − | + | − | − | − |
| Staphylococcus hyicus ATCC 11249 | − | + | − | − | − |
| Staphylococcus lutrae ATCC 700373 | − | + | − | − | − |
| Staphylococcus muscae NCTC 13833 | − | + | − | − | − |
| Staphylococcus schleiferi 1360-13 | − | + | − | − | − |
| Group iii: MpsAB only | |||||
| Staphylococcus equorum KS1039 | + | − | − | − | − |
| Staphylococcus haemolyticus JCSC1435 | + | − | − | − | − |
| Staphylococcus hominis subsp. hominis K1 | + | − | − | − | − |
| Staphylococcus nepalensis JS1 | + | − | − | − | − |
| Staphylococcus pasteuri SP1 | + | − | − | − | − |
| Staphylococcus succinus 14BME20 | + | − | − | − | − |
| Staphylococcus warneri SG1 | + | − | − | − | − |
| Group iv: CA and Ica | |||||
| Staphylococcus condimenti DSM 11674 | − | + | + | + | + |
| Staphylococcus pettenkoferi FDAARGOS_288 | − | + | +c | + | + |
| Staphylococcus piscifermentans NCTC 13836 | − | + | + | + | + |
| Staphylococcus pseudintermedius ED99 | − | + | + | + | + |
| Staphylococcus stepanovicii NCTC 13839 | − | + | + | + | + |
| Staphylococcus simulans FDAARGOS_124 | − | + | + | − | + |
The presence of the proteins was inferred based on the following Pfam domain searches from finished bacterial genomes in the Integrated Microbial Genomes & Microbiomes (IGM/G) database: MpsAB was from Pfam00361 and PFam10070, respectively, carbonic anhydrase (CA) was based on prokaryotic type-carbonic anhydrase (Pfam00484) and eukaryotic-type CA (Pfam00194), and the biofilm-associated proteins are based on the Ica-encoding genes icaA, which has the domain glycosyl transferase family 2 (Pfam00535), icaB, which contains the domain polysaccharide deacetylase (Pfam01522), and icaC, which has the domain acyltransferase family (Pfam01757). The symbols + and − indicate the presence or absence, respectively, of the protein domains. The symbol +/− indicates the presence or absence of the protein domains, with + indicates one and ++ indicate two protein domains. The symbol (+) indicates that in S. sciuri SNUSD-18, MpsA and MpsB appear to be truncated.
Two sets of operons of ica genes in different locations were detected in the genome of S. saprophyticus 883.
icaA was found to contain Pfam13641 which is annotated as glycosyltransferase-like family 2, but the protein has 76% similarity (BLASTp) to the IcaA from S. aureus.