TABLE 2.
Screening for nutritional dependence, molecular typing, and thyA mutations in thymidine-dependent and revertant Staphylococcus aureus SCV isolates
| Patient | Isolate IDa | Agar/disk supplementationb |
Phenotype |
thyA sequencing |
Genotypec | Multilocus sequence typingd |
||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| WS | THY | HEM | MEN | Synonymous mutation | Nonsynonymous mutation | Alteration(s) | Clonal complex | Sequence type | ||||
| P1 | Sa1 | −/− | +/+ | −/− | −/− | sSCV | A45G | A617G | Missense nonconservative mutation (Gln206Arg) | Revertant | 5 | 5 |
| P2 | Sa2 | −/− | +/+ | −/− | −/− | sSCV | A183G, C219T, A237G, T411A, A438T, T450G, T501A, A516G, T582C, C609A, C837T, C864T, T876A, A924C | Silent point mutations | WT | NP | NP | |
| P3 | Sa3 | −/− | +/+ | −/− | −/− | sSCV | T47G | Missense conservative mutation (Val16Gly) | sSCV | 1 | 2383 | |
| Δ48TTTAGAAATAGG59 | Frameshift mutation (−12 nte from amino acid 17) | |||||||||||
| P4 | Sa4 | −/− | +/+ | −/− | −/− | sSCV | A45G | Silent point mutation | sSCV | 5 | 221 | |
| CAACTT115CAACTCTT | Frameshift mutation (+2 nt from amino acid 40) | |||||||||||
| P5 | Sa5 | −/− | +/+ | −/− | −/− | sSCV | A55C | Missense conservative mutation (Ile19Leu) | sSCV | 398 | 398 | |
| A60G, C219T, A237G, T411A, A456G, A516G, T582C | Silent point mutations | |||||||||||
| Δ590CACTTCCGCCTT601 | Frameshift mutation (−12 nt from amino acid 197) | |||||||||||
| P6 | Sa6 | −/− | +/+ | −/− | −/− | sSCV | Δ589GCACTTCCGCCTT601 | Frameshift mutation (−13 nt from amino acid 197) | sSCV | 5 | 5 | |
| P7 | Sa7 | −/− | +/+ | −/− | −/− | sSCV | G99A | A650G | Missense nonconservative mutation (Gln217Arg) | Revertant | 1 | 188 |
| P8 | Sa8 | −/− | +/+ | −/− | −/− | sSCV | T489C, A510G, A516G | Silent point mutations | WT | NP | NP | |
| P9 | Sa9 | −/− | +/+ | −/− | −/− | sSCV | A55C | Missense conservative mutation (Ile19Leu) | sSCV | 398 | 398 | |
| A60G, A147T, C219T, A237G, T411A, A456G, A516G, T582C, A591T, A744C | Silent point mutations | |||||||||||
| Δ766ATACATTTGGAG778 | Frameshift mutation (−12 nt from amino acid 256) | |||||||||||
| P10 | Sa10 | −/− | +/+ | −/− | −/− | sSCV | Δ591ACTTCCGCCTT601 | Frameshift mutation (−11 nt from amino acid 198) | sSCV | NT | NT | |
| P11 | Sa11 | −/− | +/+ | −/− | −/− | sSCV | A192T | Missense non−conservative mutation (Leu64Phe) | sSCV | 1 | 1 | |
| Δ197G198 | Frameshift mutation (−1 nt from amino acid 66) | |||||||||||
| P12 | Sa12 | w/w | w/w | w/w | w/w | Revertant | A183G, C219T, A237G, T411A, A456G, A516G, C609A, C627T, A720G, C870T, C882T | Silent point mutations | WT | NP | NP | |
| P13 | Sa13 | w/w | w/w | w/w | w/w | Revertant | AAA451TAA | Nonsense mutation (Lys150Stop) | sSCV | NT | NT | |
| P14 | Sa14 | w/w | w/w | w/w | w/w | Revertant | A183G, C219T, A237G, T411A, A456G, A516G, C609A, C627T, A720G, C870T, C882T | Silent point mutations | WT | NP | NP | |
| P15 | Sa15 | w/w | w/w | w/w | w/w | Revertant | No mutation | WT | NP | NP | ||
| P16 | Sa16 | w/w | w/w | w/w | w/w | Revertant | G99A | Silent point mutations | WT | NP | NP | |
| P7 | Sa17 | w/w | w/w | w/w | w/w | Revertant | G99A, A282C, T495A | T495A | Missense conservative mutation (Asp165Glu) | Revertant | NP | NP |
| P8 | Sa18 | w/w | w/w | w/w | w/w | Revertant | T489C, A510G, A516G | Silent point mutations | WT | NP | NP | |
a
ID, identifier; Sa7 and Sa17 were isolated from the same patient; Sa8 and Sa18 were also isolated from the same patient (a different one).
b
WS, without supplementation; THY, thymidine; HEM, hemin; MEN, menadione; w, weak growth.
c
WT, wild type.
d
NT, nontypeable; NP, analysis not performed.
e
nt, nucleotide(s).