Table 2.

Standard curve summaries for RT-qPCR SARS-CoV-2 variant detection and the CDC N1 assay. PCR efficiency and y-intercept cycle threshold (Ct) values were calculated for each of the primer-probe sets against tenfold dilutions of the corresponding DNA oligonucleotides. N1 was tested with both the WT and B.1.1.7 oligonucleotides.

	Sdel WT	Sdel V	SN501Y WT	SN501Y V	ND3L WT	N1 (WT template)	ND3L V	N1 (V template)
Slope	−3.43	−3.36	−3.56	−3.39	−3.47	−3.57	−3.28	−3.34
Intercept	38.60	37.88	39.70	38.76	38.26	36.65	37.48	35.75
RSQ	1.000	0.996	0.994	0.999	0.997	1.000	0.999	0.998
Efficiency	95.54	98.40	90.89	97.16	94.27	90.48	101.67	99.28