Fig. 5. TPL inhibited the activation of NLRP3 inflammasome in MF rats.

(A–D) RT-PCR and WB were used to detect the mRNA and protein levels of NLRP3 and ASC in MF rats; (E) The expressions of NLRP3 and ASC were detected by immunohistochemistry (×200); n = 6. Three independent repeated tests were performed and the data were expressed as mean ± SD; one-way ANOVA was used for variance analysis; Tukey’s multiple comparisons test was used for post-hoc test. TPL, triptolide; MF, myocardial fibrosis; WB, Western blot; DMSO, dimethyl sulfoxide. Compared with sham group, **p < 0.01; compared with MF group, ^##p < 0.01.