Skip to main content
. 2021 Sep 17;17(5):1042–1050. doi: 10.4103/1673-5374.324848

Figure 1.

Figure 1

Experimental model - unilateral myelin loss localizes to FG-postive regions 7 days post LPC-induced demyelination of the right dorsal columns.

(A) CNS focal demyelination model +/- delayed electrical stimulation (ES) - at 7 days post-LPC. (B) Transverse right dorsal column section 7 days post LPC/FG injection showing paucity of MBP immunofluorescence (IF) in FG-postive region (arrows). (C) L5 DRG section showing retrograde transport of FG (blue) by L5 sensory afferents. Thereby assuring correct level of the dorsal columns was affected by sciatic nerve electrical stimulation. Note that only large size neurons appear to be FG-postive and only a single large calcitonin gene-related peptide (CGRP) IF-postive neuron (pink) is dual labelled, as would be expected for myelinated axons within the dorsal columns affected by the LPC/FG injection. (D) Longitudinal section of 7 day LPC-focally demyelinated right dorsal columns processed for dual ED-1 and MBP IF reveals loss of myelin (MBP) area coincides with FG intense area and region of activated macrophages/microglia as detected by the ED-1/MBP IF. Merged images of the ED-1 (green) and MBP (red) IF (below) demonstrate the localization of activated macrophages/microglia to regions of myelin loss. Scale bars: 100 μm for B–D. FG: Fluorogold; LPC: lysophosphatidyl choline; MBP: myelin basic protein.