TABLE 3.
List of PCR primers used in this study
| Initial construct | PCR primer sequence (5′–3′) | PCR product |
|---|---|---|
| pGEM7Z_dxnB1 | GGGGAATTCCGAAAGGCGCTCACTTCGAGGAC | dxnB1 with flanking regions for knockout construction |
| GGGAATTCGAAGTTGCCGTGACACCG | dxnB1 with flanking regions for knockout construction | |
| pGEMT_dxnB2 | GGAAGCTTCTGGGTCACGCCTGCTTCG | dxnB2 with flanking regions for knockout construction |
| GGTCTAGACCTAGCAGCTTGCCGTCATG | dxnB2 with flanking regions for knockout construction | |
| pET_0910 | GGAAGCTTGCAACATCGTCCTGGTCG | SWIT0910 with flanking regions for knockout construction |
| GGAATTCGCAGGGCATAAGCGACGCAGTC | SWIT0910 with flanking regions for knockout construction | |
| pGEMT_0886 | GGTTCTAGACCCAGGGCGACCGGCTATGTC | SWIT0886 for complementation |
| GAATTCGACGATGGCGGTCTTCATCGCG | SWIT0886 for complementation | |
| pCR_dxnB1 | GGGAATTCGGGGAATCGTGAGGATAGAAATGACCCAGC | dxnB1 for complementation |
| CCCAAGCTTGCATGCTAGAATTTCCGAGCG | dxnB1 for complementation | |
| pCR_dxnB2 | GTCGACGACGGCATTGCCGGTCGGTG | dxnB2 for complementation |
| AAGCTTCGGCCATCGATCAATCCAGC | dxnB2 for complementation | |
| pCR_0910 | GAATTCGGAGGACGGATTGGGGATC | SWIT0910 for complementation |
| AAGCTTATCGCTGGCGAGGGGAGGAT | SWIT0910 for complementation |