TABLE 1.
Transposon mutants of B. cepacia complex strain R-12632 with loss of or reduced inhibitory activity against K. pneumoniae LMG 2095a
| Disrupted gene | Length (bp) | Mutant(s) (insertion site) | Name | Putative function | EC no. | KO no. | Pathway |
|---|---|---|---|---|---|---|---|
| Mutants with loss of inhibitory activity | |||||||
| BCCR12632_00250 | 1089 | R-75501 (892) | paaE | Ring-1,2-phenylacetyl-CoA epoxidase subunit E | 1.14.13.149 | K02613 | Phenylacetate catabolism |
| BCCR12632_00471 | 792 | R-75722 (212), R-75499 (212) | paaG | Enoyl-CoA isomerase | 5.3.3.18 | K15866 | Phenylacetate catabolism |
| BCCR12632_02545 | 1317 | R-75718 (741) | cysN | ATP sulfurylase subunit 1 | 2.7.7.4 | K00956 | Assimilatory sulfate reduction |
| BCCR12632_02546 | 963 | R-75500 (361) | cysD | ATP sulfurylase subunit 2 | 2.7.7.4 | K00957 | Assimilatory sulfate reduction |
| BCCR12632_02547 | 747 | R-75588 (664), R-75389 (664) | cysH | APS reductase | 1.8.4.10 | K00390 | Assimilatory sulfate reduction |
| BCCR12632_02549 | 1680 | R-75388 (1539) | cysI | Sulfite reductase | 1.8.1.2 | K00381 | Assimilatory sulfate reduction |
| BCCR12632_02955 | 957 | R-75384 (903), R-75390 (444), R-75385 (477), R-75386 (300), R-75387 (932) | gshB | Glutathione synthetase | 6.3.2.3 | K01920 | Glutathione metabolism |
| BCCR12632_00396 | 1836 | R-75587 (1302) | tssF | Type VI secretion protein | NA | K11896 | Type VI secretion system |
| BCCR12632_00424 | 843 | R-75502 (669) | hemK | N5-glutamine S-adenosyl-l-methionine-dependent methyltransferase |
2.1.1.297 | K02493 | Unknown |
| Mutants with reduced inhibitory activity | |||||||
| BCCR12632_02540 | 1512 | R-75714 (959), R-75715 (959), R-75719 (434), R-75720 (959) | pepA | Cytosol aminopeptidase | 3.4.11.1 | K01255 | Glutathione metabolism |
| BCCR12632_05920 | 987 | R-75599 (799), R-75602 (799), R-75716 (858), R-75717 (858), R-75721 (858) | mdh | Malate dehydrogenase | 1.1.1.37 | K00024 | Energy metabolism |
| BCCR12632_02740 | 882 | R-75596 (496) | sucD | Succinyl-CoA synthetase subunit alpha | 6.2.1.5 | K01902 | Energy metabolism |
| BCCR12632_02305 | 651 | R-75593 (614) | nuoJ | NADH:ubiquinone oxidoreductase subunit J | 7.1.1.2 | K00339 | Energy metabolism |
| BCCR12632_00598 | 1287 | R-75594 (745), R-75600 (227), R-75601 (1077), R-75723 (1258) | gdhA | Glutamate dehydrogenase | 1.4.1.4 | K00262 | Energy metabolism |
| BCCR12632_00209 | 459 | R-75724 (358), R-75726 (343) | asnC | AsnC family transcriptional regulator | NA | K03718 | Unknown |
| BCCR12632_02888 | 909 | R-75597 (702), R-75598 (837) | rapZ | RNase adapter protein | NA | K06958 | Unknown |
| BCCR12632_03045 | 3573 | R-75725 (3153) | iorA | 2-Oxoacid ferredoxin oxidoreductase | 1.2.7.8 | K00179 | Unknown |
| BCCR12632_03202 | 543 | R-75592 (300) | regA | Two-component system, response regulator | NA | K15012 | Unknown |
| BCCR12632_03317 | 885 | R-75595 (630) | GGPS | Geranylgeranyl diphosphate synthase | 2.5.1.1 | K13789 | Unknown |
a
Mutants indicated in bold type were selected for comparative metabolite analysis. KO, KEGG Orthology; APS, adenosine 5′-phosphosulfate; NA, not available; CoA, coenzyme A.