TABLE 3.
Metabolites produced by transformation of HOCDA (V in Fig. 1) by P. stutzeri Chol1 ΔkshA complemented with the given genes on vector pBBR1MCS-5 after 6 days as determined by HPLC-MS and identified by retention time, mass, and UV-spectruma
| Gene in strain Chol1 ΔkshA | Products formed from HOCDA |
|---|---|
| kshAChol1 | DHSATD (XI in Fig. 1), THADD (XXIII) |
| nov2c066 | HATD (X), 12β-DHADD (VI) |
| nov2c228 | HATD, 12β-DHADD, other (Fig. 9) |
| nov2c407 | HATD, 12β-DHADD, DHSATD |
| nov2c430 | HATD, 12β-DHADD, DHSATD |
| nov2c440 | HATD, 12β-DHADD, DHSATD |
| Empty vector | HATD, 12β-DHADD |
a
THADD, 1,2,12-trihydroxy-androsta-4,6-diene-3,12-dione (XXIII in Fig. S13).