. Author manuscript; available in PMC: 2021 Oct 28.

Published in final edited form as: Evol Dev. 2021 Aug 19;23(5):404–422. doi: 10.1111/ede.12387

Table 1.

Number of cells expressing particular genes in WT and lbx1 mutant embryos.

	slc32a1	slc32a1	slc17a6	slc17a6	gads	slc6a5
Figure 5 Panels	a, b, m	c, d, n	e, f, o	g, h p	i, j, q	k, l, r
Stage	24h	30h	30h	30h	30h	30h
WT vs lbx1a ^−/−
Mean Number of Cells + S.E.M.	124.0 ± 3.06 vs 115.3 ± 5.27	164.6 ± 2.80 vs 131.8 ± 1.40	102.1 ± 2.28 vs 120.0 ± 2.54	107.2 ± 2.34 vs 124.2 ± 2.85	91.8 ± 4.59 vs 89.6 ± 2.04	153.3 ± 1.55 vs 145.3 ± 2.40
p-value (a)	0.379^‡	<0.001 ^‡	<0.001 ^‡	0.001 ⁺	0.966^‡	0.266^‡
WT vs lbx1b ^−/−
Mean Number of Cells + S.E.M.	124.0 ± 3.06 vs 114.0 ± 2.89	164.6 ± 2.80 vs 139.5 ± 1.76	102.1 ± 2.28 vs 106.7 ± 2.14	Not applicable	91.8 ± 4.59 vs 87.2 ± 3.62	153.3 ± 1.55 vs 142.7 ± 2.73
p-value (b)	0.188^‡	<0.001 ^‡	0.499	Not applicable	0.766^‡	0.096^‡
WT vs lbx1a ^−/− ; lbx1b ^−/−
Mean Number of Cells + S.E.M.	124.0 ± 3.06 vs 115.7 ± 3.19	164.6 ± 2.80 vs 136.8 ± 2.08	102.1 ± 2.28 vs 121.7 ± 2.11	Not applicable	91.8 ± 4.59 vs 84.8 ± 2.52	153.3 ± 1.55 vs 133.7 ± 2.81
p-value (c)	0.301^‡	<0.001 ^‡	<0.001 ^‡	Not applicable	0.471^‡	<0.001 ^‡
*lbx1a^−/− vs lbx1a^−/−; lbx1b*^−/−
Mean Number of Cells + S.E.M.	115.3 ± 5.27 vs 115.7 ± 3.19	131.8 ± 1.40 vs 136.8 ± 2.08	120.0 ± 2.54 vs 121.7 ± 2.11	Not applicable	89.6 ± 2.04 vs 84.8 ± 2.52	145.3 ± 2.40 vs 133.7 ± 2.81
p-value (d)	0.999^‡	0.392^‡	0.951^‡	Not applicable	0.743^‡	0.042 ^‡
*lbx1b^−/− vs lbx1a^−/−; lbx1b*^−/−
Mean Number of Cells + S.E.M.	114.0 ± 2.89 vs 115.7 ± 3.19	139.5 ± 1.76 vs 136.8 ± 2.08	106.7 ± 2.14 vs 121.7 ± 2.11	Not applicable	87.2 ± 3.62 vs 84.8 ± 2.52	142.7 ± 2.73 vs 133.7 ± 2.81
p-value (e)	0.982^‡	0.855^‡	<0.001 ^‡	Not applicable	0.956^‡	0.136^‡
*lbx1a^−/− vs lbx1b*^−/−
Mean Number of Cells + S.E.M.	115.3 ± 5.27 vs 114.0 ± 2.89	131.8 ± 1.40 vs 139.5 ± 1.76	120.0 ± 2.54 vs 106.7 ± 2.14	Not applicable	89.6 ± 2.04 vs 87.2 ± 3.62	145.3 ± 2.40 vs 142.7 ± 2.73
p-value (f)	0.995^‡	0.183^‡	0.001	Not applicable	0.956^‡	0.927^‡

Numbers of cells expressing particular genes (row 1) in spinal cord region adjacent to somites 6–10 and p values of comparisons between embryos with different genotypes (rows 6, 9, 12, 15, 18 and 21). Row 2 indicates the panels in Figure 5 that show the data for that comparison and row 3 indicates the developmental stage assayed. Rows 4, 7, 10, 13, 16 and 19 indicate the genotypes being compared. Values (rows 5, 8, 11, 14, 17 and 20) indicate the mean from at least 3 different embryos ± standard error of the mean (S.E.M.) for each genotype in that particular comparison. For all genes assayed in both lbx1a/b single and double mutants, a standard ANOVA was performed with Tukey’s honestly significant difference post-hoc testing. P values generated by this method of testing are indicated by (^‡). For the 30h slc17a6 experiment performed on lbx1a^hu3569 mutant embryos, a type 2 student’s t-test was performed, with p values indicated by (⁺). Statistically significant (p<0.05) values are indicated in bold. For a discussion of why particular tests were used, see materials and methods. P(a) (row 6) compares WT with lbx1a single mutant embryos, P(b) (row 9) compares WT with lbx1b single mutant embryos, P(c) (row 12) compares WT with lbx1a;lbx1b double mutant embryos, P(d) (row 15) compares lbx1a single mutant embryos with lbx1a;lbx1b double mutant embryos, P(e) (row 18) compares lbx1b single mutant embryos with lbx1a;lbx1b double mutant embryos and P(f) (row 21) compares lbx1a single mutant embryos with lbx1b single mutant embryos. Mean cell count values are provided to one decimal place, S.E.M. values to 2 decimal places and p values to three decimal places. The lbx1a^sa1496 allele was used in all experiments except the 24h slc32a1 double mutant experiment and the 30h slc17a6 single mutant experiment, in which the lbx1a^hu3659 allele was used instead. In all cases, the lbx1b allele used was lbx1b^hu3534.