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. 2021 Oct 28;16(10):e0259133. doi: 10.1371/journal.pone.0259133

Fig 4. RLS-0071 inhibits complement activation.

Fig 4

Plasma was isolated from sham animals (n = 3) and the following groups prior to first-hit (0 minutes) and at 5 minutes and 1 hour: first hit only (n = 3), 2-hit (n = 3), 2-hit + 10 mg/kg prophylactic dose RLS-0071 (n = 8), 2-hit + 40 mg/kg prophylactic dose RLS-0071 (n = 4), 2-hit + 160 mg/kg prophylactic dose RLS-0071 (n = 5), 2-hit + 40 mg/kg rescue dose RLS-0071 at 0.5 min (n = 5), 2-hit + 40 mg/kg rescue dose RLS-0071 at 60 min (n = 3), 2-hit + 40 mg/kg rescue dose RLS-0071 at 90 min (n = 5), 2-hit + 40 mg/kg rescue dose RLS-0071 at 120 min (n = 3) and 2-hit + 40 mg/kg rescue dose RLS-0071 at 180 min (n = 3). C5a was then measured in each sample by ELISA and absorbance was read at 450 nm. Two replicates for each animal were measured for every time point. Data are means and standard error of the mean. Statistical analysis was performed using were conducted using bootstrap approach or Welch’s ANOVA. * denotes p = 0.010, ** denotes p = 0.004, *** denotes p = 0.002, and **** denotes p≤0.001 compared to 2-hit animals.