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. 2021 Oct 28;16(10):e0259133. doi: 10.1371/journal.pone.0259133

Fig 5. RLS-0071 reduces free DNA levels in the blood.

Fig 5

Plasma was isolated from sham animals (n = 3) and the following groups at 4 hours after start of the experiments: first hit only (n = 3), 2-hit (n = 3), 2-hit + 10 mg/kg prophylactic dose RLS-0071 (n = 9), 2-hit + 40 mg/kg prophylactic dose RLS-0071 (n = 4), 2-hit + 160 mg/kg prophylactic dose RLS-0071 (n = 5), 2-hit + 40 mg/kg rescue dose RLS-0071 at 0.5 min (n = 4), 2-hit + 40 mg/kg rescue dose RLS-0071 at 60 min (n = 3), 2-hit + 40 mg/kg rescue dose RLS-0071 at 90 min (n = 5), 2-hit + 40 mg/kg rescue dose RLS-0071 at 120 min (n = 3) and 2-hit + 40 mg/kg rescue dose RLS-0071 at 180 min (n = 3). Plasma samples were incubated with PicoGreen. Fluorescence was read at an excitation wavelength of 485 nm and an emission wavelength of 520nm in a microplate reader. All free DNA measurements for each animal were done in triplicate. Data are means and standard error of the mean. Statistical analysis was performed using bootstrap approach or Welch’s ANOVA. *denotes p = 0.026, **denotes p = 0.039 and ***denotes p = 0.005 compared to 2-hit animals.