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. 2021 Oct 28;16(10):e0259068. doi: 10.1371/journal.pone.0259068

Fig 3. Identification of SSX2IP by targeted proximity biotinylation.

Fig 3

A, Scheme of targeted proximity biotinylation (TPB) with GFP-WtipN. Early embryos were injected with indicated RNAs (200 pg each) and biotin (1.2 mM). B, Biotinylated proteins were enriched with neutravidin agarose beads and immunoblotted with indicated antibodies. Biotinylated GFP-WtipN (asterisk) but not Flag-WtipN in the presence of BirA-GBP was detected in the pulldown. MycBirA-WtipN (asterisk) was biotinylated in the absence of BirA-GBP. C, Top hits identified by SCAFFOLD after LC-MS/MS analysis in both experimental groups but not in the negative control group.