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. 2021 Oct 14;12:718005. doi: 10.3389/fimmu.2021.718005

Figure 1.

Figure 1

Macrophages are the major immune cells in the E18.5 skin and are derived from the circulatory monocytes. Increase in the macrophage population and BM disruption in the Itgβ1 epidermal KO skin compared to the control skin depicted by the immunostaining for F4/80, Laminin 332 (Lam-332), and Keratin 5 (K5) at E17.5 (A, B) and E18.5 (C, D). Immunostaining of F4/80 cells with Ki67 in the control and the Itgβ1 KO skin at E17.5 (E, F) and E18.5 (G, H). White dashed lines mark the dermal (Der)-epidermal (Epi) separation. Cell cycle analysis at E17.5 and E18.5 in the control and the KO embryonic skin (I–L). Bar graphs representing percentage of F4/80 cells in the E17.5 and E18.5 control and the KO skin (M) (N=2). Quantification of the flow cytometry data in the E17.5 and E18.5 control and tgβ1 epidermal KO skin for the expression of F4/80+CD11B+ (N) and F4/80+LY6C+ (O) in sorted CD45+ cells (N=3). Quantification of the normalized geometric mean fluorescence intensity (GMFI) expression of F4/80, CD11B, and LY6C on the macrophages in the E18.5 compared to the E17.5 KOs (P) (N=3). Scale bars: 20 µm. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ns=not significant.