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. 2021 Oct 14;12:718005. doi: 10.3389/fimmu.2021.718005

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Copyright © 2021 Bhattacharjee, Ayyangar, Kurbet, Lakshmanan, Palakodeti, Ginhoux and Raghavan

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Macrophages in the KO skin acquire exaggerated M2-like pro-remodelling properties at E18.5. Immunostaining in the control and Itgβ1 epidermal KO skin at E17.5 and E18.5 for F4/80 and MERTK (A–D) quantified in (E) (N=3). Surface level expression of MERTK is quantified (F) (N=3). Immunostaining in the control and Itgβ1 epidermal KO skin at E17.5 and E18.5 for F4/80 and Arg1 (G–J) quantified in (K) (N=2). Immunostaining in the control and Itgβ1 epidermal KO skin at E17.5 and E18.5 for F4/80 and FN1 (L–O) quantified in (P) (N=2). Immunostaining in the control and Itgβ1 epidermal KO skin at E17.5 and E18.5 for F4/80 and MMP9 (Q–T) quantified in (U) (N=2). The white dashed line separates the epidermis (Epi) and the dermis (Der). Scale bars: 20 um. (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001, ns=not significant).