| Antibody |
Anti-mouse CD3ε-PerCP (clone: 145–2 C11); (Armenian hamster monoclonal) |
BioLegend |
Cat No#100326; RRID: AB_893317
|
FACS (1:100) |
| Antibody |
Anti-mouse CD4-APC (clone: GK1.5); (rat monoclonal) |
BioLegend |
Cat No#100412; RRID: AB_312697
|
FACS (1:100) |
| Antibody |
Anti-mouse CD4-PE (clone: GK1.5); (rat monoclonal) |
BioLegend |
Cat No#100408; RRID: AB_312693
|
FACS (1:100) |
| Antibody |
Anti-mouse CD4-Pacific Blue (clone: GK1.5); (rat monoclonal) |
BioLegend |
Cat No#100428; RRID: AB_ 493,647 |
FACS (1:100) |
| Antibody |
Anti-mouse CD8a-FITC (clone: 53–6.7); (rat monoclonal) |
BioLegend |
Cat No#100706; RRID: AB_312745
|
FACS (1:100) |
| Antibody |
Anti-mouse CD8a-Pacific Blue(clone: 53–6.7); (rat monoclonal) |
BioLegend |
Cat No#100725; RRID: AB_493425
|
FACS (1:100) |
| Antibody |
Anti-mouse CD25-BV711 (clone: PC61); (rat monoclonal) |
BioLegend |
Cat No#102049; RRID: AB_2564130
|
FACS (1:100) |
| Antibody |
Anti-mouse CD11b-Pacific Blue (clone: M1/70); (rat monoclonal) |
BioLegend |
Cat No#101224; RRID: AB_755986
|
FACS (1:100) |
| Antibody |
Anti-mouse CD11c-PE-Cy7 (clone: N418); (Armenian hamster monoclonal) |
BioLegend |
Cat No#117318; RRID: AB_493568
|
FACS (1:100) |
| Antibody |
Anti-mouse CD25-PE-Cy7 (clone: PC61); (rat monoclonal) |
BioLegend |
Cat No#102016; RRID: AB_312865
|
FACS (1:100) |
| Antibody |
Anti-mouse CD40L-APC (clone: MR1); (Armenian hamster monoclonal) |
BioLegend |
Cat No#106510; RRID: AB_2561561
|
FACS (1:100) |
| Antibody |
Anti-mouse CD44-PE-Cy7 (clone: IM7); (rat monoclonal) |
BioLegend |
Cat No#103030; RRID: AB_830787
|
FACS (1:100) |
| Antibody |
Anti-mouse CD45.2-PE-Cy7 (clone: 104); (mouse monoclonal) |
BioLegend |
Cat No#109830; RRID: AB_1186098
|
FACS (1:100) |
| Antibody |
Anti-mouse CD71-APC (clone: RI7217); (rat monoclonal) |
BioLegend |
Cat No#113820; RRID: AB_2728135
|
FACS (1:100) |
| Antibody |
Anti-mouse CD98-PE-Cy7 (clone: RL388); (rat monoclonal) |
BioLegend |
Cat No#128214; RRID: AB_2750547
|
FACS (1:100) |
| Antibody |
Anti-mouse CD45.2-PerCP (clone: 104); (mouse monoclonal) |
BioLegend |
Cat No#109826; RRID: AB_893349
|
FACS (1:100) |
| Antibody |
Anti-mouse CD62L-Pacific Blue (clone: MEL-14); (rat monoclonal) |
BioLegend |
Cat No#104424; RRID: AB_493380
|
FACS (1:100) |
| Antibody |
Anti-mouse CD69-PE (clone: H1.2F3); (Armenian hamster monoclonal) |
BioLegend |
Cat No#104508; RRID: AB_313111
|
FACS (1:100) |
| Antibody |
Anti-mouse CD107a-APC-Cy7 (clone: 1D4B); (rat monoclonal) |
BioLegend |
Cat No#121616; RRID: AB_10643268
|
FACS (1:100) |
| Antibody |
Anti-mouse Foxp3-PE (clone: MF-14); (rat monoclonal) |
BioLegend |
Cat No#126404; RRID: AB_1089117
|
FACS (1:100) |
| Antibody |
Anti-mouse siglec-F (CD170)-APC (clone: S17007L); (rat monoclonal) |
BioLegend |
Cat No#155508; RRID: AB_2750237
|
FACS (1:100) |
| Antibody |
Anti-mouse Ly6C-APC (clone: HK1.4); (rat monoclonal) |
BioLegend |
Cat No#128016; RRID: AB_1732076
|
FACS (1:100) |
| Antibody |
Anti-mouse Ly6G-PE (clone: 1A8); (rat monoclonal) |
BioLegend |
Cat No#127608; RRID: AB_1186099
|
FACS (1:100) |
| Antibody |
Anti-mouse IL-4-PE (clone: 11B11); (rat monoclonal) |
BioLegend |
Cat No#504104; RRID: AB_315318
|
FACS (1:100) |
| Antibody |
Anti-mouse IL-5-APC (clone: TRFK5); (rat monoclonal) |
BioLegend |
Cat No#504306; RRID: AB_315330
|
FACS (1:100) |
| Antibody |
Anti-mouse IL-13-PE (clone: eBio13A); (rat monoclonal) |
ThermoFisher |
Cat No# 12-7133-82; RRID: AB_763559
|
FACS (1:100) |
| Antibody |
Anti-mouse IL-17A-PE (clone: eBio17B7); (rat monoclonal) |
ThermoFisher |
Cat No#12-7177-81; RRID: AB_763582
|
FACS (1:100) |
| Antibody |
Anti-mouse IFN-γ-APC (clone: XMG1.2); (rat monoclonal) |
BioLegend |
Cat No#505810; RRID: AB_315404
|
FACS (1:100) |
| Antibody |
Anti-mouse Granzyme B-PE (clone: NGZB); (rat monoclonal) |
ThermoFisher |
Cat No#12-8898-82; RRID: AB_10870787
|
FACS (1:100) |
| Antibody |
Anti-mouse CD3ε-Purified (clone: 145–2 C11); (Armenian hamster monoclonal) |
BioLegend |
Cat No#100340; RRID: AB_11149115
|
Culture (1:1000) |
| Antibody |
Anti-mouse CD28-Purified (clone: 37.51); (Syrian hamster monoclonal) |
BioLegend |
Cat No#102116; RRID: AB_11147170
|
Culture (1:1000) |
| Antibody |
Anti-mouse 6PGD; (rabbit polyclonal) |
Sigma Aldrich |
Cat No#HPA031314; RRID: AB_10610278
|
Western blot (1:1000) |
| Antibody |
Anti-β-actin (D6A8); (rabbit monoclonal) |
Cell Signaling Technology |
Cat No#8457; RRID: AB_10950489
|
Western blot (1:1000) |
| Genetic reagent (species) |
Pgd TaqMan Assay probe (FAM-MGB) (mouse) |
ThermoFisher |
Cat No#4351372; Assay ID:Mm01263703_m1 |
|
| Genetic reagent (species) |
Foxp3 TaqMan Assay probe (FAM-MGB) (mouse) |
ThermoFisher |
Cat No#4331182; Assay ID: Mm00475162_m1 |
|
| Genetic reagent (species) |
Gata3 TaqMan Assay probe (FAM-MGB) (mouse) |
ThermoFisher |
Cat No#4331182; Assay ID:Mm00484683_m1 |
|
| Genetic reagent (species) |
Il5 TaqMan Assay probe (FAM-MGB) (mouse) |
ThermoFisher |
Cat No#4331182; Assay ID:Mm00439646_m1 |
|
| Genetic reagent (species) |
Il13 TaqMan Assay probe (FAM-MGB) (mouse) |
ThermoFisher |
Cat No#4331182; Assay ID: Mm00434204_m1 |
|
| Genetic reagent (species) |
Ifng TaqMan Assay probe (FAM-MGB) (mouse) |
ThermoFisher |
Cat No#4331182; Assay ID: Mm01168134_m1 |
|
| Genetic reagent (species) |
Tbet (Tbx21) TaqMan Assay probe (FAM-MGB) (mouse) |
ThermoFisher |
Cat No# 4331182; Assay ID: Mm00450960_m1 |
|
| Genetic reagent (species) |
Il17a TaqMan Assay probe (FAM-MGB) (mouse) |
ThermoFisher |
Cat No#4331182; Assay ID: Mm00439618_m1 |
|
| Genetic reagent (species) |
Icos TaqMan Assay probe (FAM-MGB) |
ThermoFisher |
Cat No#4331182; Assay ID: Mm00497600_m1 |
|
| Genetic reagent (species) |
Il4 TaqMan Assay probe (FAM-MGB) (mouse) |
ThermoFisher |
Cat No#4331182; Assay ID:Mm00445259_m1 |
|
| Genetic reagent |
Il10 TaqMan Assay probe (FAM-MGB) (mouse) |
ThermoFisher |
Cat No#4331182; Assay ID: Mm00439614_m1 |
|
| Genetic reagent (species) |
Batf3 TaqMan Assay probe (FAM-MGB) (mouse) |
ThermoFisher |
Cat No#4331182; Assay ID: Mm01318274_m1 |
|
| Genetic reagent (species) |
Hey1 TaqMan Assay probe (FAM-MGB) (mouse) |
ThermoFisher |
Cat No#4331182; Assay ID: Mm00468865_m1 |
|
| Genetic reagent (species) |
Bcl6 TaqMan Assay probe (FAM-MGB) (mouse) |
ThermoFisher |
Cat No#4331182; Assay ID: Mm00477633_m1 |
|
| Genetic reagent (species) |
Gzb TaqMan Assay probe (FAM-MGB) (mouse) |
ThermoFisher |
Cat No#4331182; Assay ID: Mm00442834_m1 |
|
| Genetic reagent (species) |
18 S rRNA TaqMan Assay probe (VIC-MGB) (mouse) |
ThermoFisher |
Cat No#4319413E |
|
| Cell line (species) |
B16-F10 (mouse) |
ATCC |
ATCC CRL-6475; RRID:CVCL_0159
|
|
| Chemical compound, drug |
6-Aminonicotinamide (6-AN) |
Sigma Aldrich |
Cat No#A68203 |
|
| Chemical compound, drug |
Tamoxifen |
Sigma Aldrich |
Cat No#T5648-1G |
|
| Chemical compound, drug |
Dehydroepiandrosterone (DHEA) |
Cayman Chemical |
Cat No#15728 |
|
| Chemical compound, drug |
Dimethyl sulfoxide (DMSO) |
Sigma Aldrich |
Cat No#D2438 |
|
| Chemical compound, drug |
Tetra-methylrhodamine ester (TMRE) |
ThermoFisher |
Cat No#T669 |
|
| Chemical compound, drug |
MitoSOX Red |
ThermoFisher |
Cat No#M36008
|
|
| Chemical compound, drug |
MitoTracker Deep Red FM |
ThermoFisher |
Cat No#M22426
|
|
| Chemical compound, drug |
2-NBD-glucose (2-NBDG) |
Cayman Chemical |
Cat No#11046 |
|
| Chemical compound, drug |
D-GLUCOSE (1,2,3,4,5,6,6-D7, 97–98%) |
Cambridge Isotope laboratories |
Cat No#DLM-2062-PK |
|
| Chemical compound, drug |
13C5,15N2-Glutamine |
Cambridge Isotope laboratories |
Cat No#CNLM-1275-H-PK |
|
| Chemical compound, drug |
Fetal bovine serum, heat inactivated |
ThermoFisher |
Cat No#16140071 |
|
| Chemical compound, drug |
NuPAGE 4–12%, Bis-Tris, 1.5 mm, Mini Protein Gel |
ThermoFisher |
Cat No#NP0335BOX |
|
| Chemical compound, drug |
NuPAGE MES SDS Running Buffer |
ThermoFisher |
Cat No#NP0002 |
|
| Chemical compound, drug |
Recombinant Mouse IL-2 |
BioLegend |
Cat No#575404 |
|
| Chemical compound, drug |
Recombinant Mouse TGF-β1 |
BioLegend |
Cat No#763104 |
|
| Commercial assay or kit |
Milliplex MAP Mouse Cytokine/Chemokine Magnetic Bead Panel – Premixed 32 Plex |
Millipore |
Cat No# MCYTMAG-70K-PX32 |
|
| Commercial assay or kit |
Mouse Ig Isotyping Array Q1 |
RayBiotech |
Cat No#QAM-ISO-1–2 |
|
| Commercial assay or kit |
CellTrace CFSE Cell Proliferation Kit |
ThermoFisher |
Cat No#C34554
|
|
| Commercial assay or kit |
Dynabeads Mouse T-Activator CD3/CD28 |
ThermoFisher |
Cat No#11456D |
|
| Commercial assay or kit |
Fixation/Permeabilization Solution Kit with BD GolgiPlug |
BD Bioscience |
Cat No#555028 |
|
| Commercial assay or kit |
eBioscience Foxp3/ Transcription Factor Staining Buffer Set |
ThermoFisher |
Cat No#00-5523-00 |
|
| Commercial assay or kit |
LIVE/DEAD Fixable Aqua Dead Cell Stain Kit (Aqua) |
ThermoFisher |
Cat No#L34957
|
|
| Commercial assay or kit |
EasySep Mouse CD4+ T Cell Isolation Kit |
STEMCELL Technologies |
Cat No#19852RF |
|
| Commercial assay or kit |
EasySep Mouse Naïve CD4+ T Cell Isolation Kit |
STEMCELL Technologies |
Cat No#19765 |
|
| Commercial assay or kit |
EasySep Mouse CD4+ CD25+ Regulatory T Cell Isolation Kit II |
STEMCELL Technologies |
Cat No#18783 |
|
| Commercial assay or kit |
RNeasy Mini Kit |
Qiagen |
Cat No#74104 |
|
| Commercial assay or kit |
Seahorse XF Cell Mito Stress Test Kit |
Agilent |
Cat No#103015–100 |
|
| Commercial assay or kit |
Pierce BCA Protein Assay Kit |
ThermoFisher |
Cat No#23225 |
|
| Genetic reagent(Mus musculus) |
Pgdfl/fl Foxp3YFP-Cre |
This paper |
N/A |
6PGD exon 5 floxed. Send reagent request to pseth@bidmc.harvard.edu
|
| Genetic reagent(Mus musculus) |
C57BL/6 J (B6 CD45.2+) |
The Jackson Laboratory |
Stock No: 000664 |
|
| Genetic reagent(Mus musculus) |
B6.SJL-Ptprca Pepcb/BoyJ (B6 CD45.1+) |
The Jackson Laboratory |
Stock No: 002014 | B6 Cd45.1 |
|
| Genetic reagent(Mus musculus) |
B6.129S7-Rag1tm1Mom/J |
The Jackson Laboratory |
Stock No: 002216 |
|
| Genetic reagent(Mus musculus) |
B6.129(Cg)-Foxp3tm4(YFP/icre)Ayr/J |
The Jackson Laboratory |
Stock No: 016959 |
|
| Genetic reagent(Mus musculus) |
Foxp3tm9(EGFP/cre/ERT2)Ayr/J |
The Jackson Laboratory |
Stock No: 016961 |
|
| Software, algorithm |
FlowJo_V10 |
FlowJo |
https://www.flowjo.com/; RRID:SCR_008520
|
|
| Software, algorithm |
GraphPad Prism_V6 |
GraphPad |
https://www.graphpad.com/; RRID:SCR_002798
|
|
