Skip to main content
. 2021 Oct 11;10:e68761. doi: 10.7554/eLife.68761

Figure 5. Whole blood clot contraction.

Whole blood clots were prepared from whole blood from Fga-/- non-activated mice supplemented with either truncated fibrinogen or WT, no fibrinogen (controls). (A) Representative images of whole blood clot formation and contraction over time, showing the formation of a defined clot for WT and α390, but no visible clotting for α220 and controls. (B) Whole blood clot contraction kinetics was similar between α390 and WT, but not quantifiable for α220. (C) Final clot weight was not different between α390 and WT, but not quantifiable for α220. (D) The percentage of red blood cells incorporated in the contracted clot was similar between α390 and WT, whilst that of α220 was similar to the control containing tissue factor but no fibrinogen. Results shown as mean ± SD, n = 3, *p<0.05 by one-way ANOVA with Dunnett’s multiple comparison test relative to the tissue factor-activated control.

Figure 5—source data 1. Retraction data (Figure 5B), weights (Figure 5C), and absorbances for red blood cell incorporation (Figure 5D).

Figure 5.

Figure 5—figure supplement 1. Clot incorporation of platelets.

Figure 5—figure supplement 1.

(A) Representative flow cytometry scatter plots showing matching samples taken before activation (non-activated blue) and post-activated (activated unclotted sample, orange) from the clot contraction experiment (Figure 5). (B) Number of platelets included in the retracted clot was significantly reduced in α220, but not α390, compared to WT. Platelet incorporation was reduced and unchanged for without fibrinogen, and without fibrinogen and tissue factor, controls, respectively. Results shown as mean ± SD, n = 3, **p<0.01 by two-way ANOVA with Sidak’s multiple comparison test non-activated samples and activated samples.
Figure 5—figure supplement 2. Recombinant fibrinogen is able to bind to platelets.

Figure 5—figure supplement 2.

(A) Percentage of fibrinogen-positive platelets measured by flow cytometry in whole blood from Fga-/- mice supplemented with truncated, WT, or γ′γ′ fibrinogens. Positivity was unchanged for α390 and α220, but significantly reduced in γ′γ′, compared to WT in activated platelets. Basal platelet positivity was unchanged across all fibrinogen variants. (B) Fibrinogen median fluorescence intensity (MFI) per platelet was significantly decreased for α220 and γ′γ′, but not for α390, compared to WT, in activated platelets. Basal platelet MFI was unchanged across all fibrinogen variants. Results shown as mean ± SD, n = 3, *p<0.05, **p<0.01, ****p<0.0001 by two-way ANOVA with Dunnett’s multiple comparison test relative to WT for each agonist.