| Genetic reagent (Mus musculus) |
Fga-/-
|
Kind gift from Dr Jay Degen, Cincinnati Children’s Hospital Medical Centre |
|
PMID:7649481
|
| Genetic reagent (M. musculus) |
Fga+/-
|
Kind gift from Dr Jay Degen, Cincinnati Children’s Hospital Medical Centre |
|
PMID:7649481
|
| Cell line (Cricetulus griseus) |
CHO-αβγ |
Kind gift from Dr Susan Lord, University of North Carolina |
Expressing WT human fibrinogen |
PMID:8418831
|
| cell line (C. griseus) |
CHO-βγ |
Kind gift from Dr Susan Lord, University of North Carolina |
Expressing β- and γ-chain of human fibrinogen |
PMID:8418831
|
| Cell line (C. griseus) |
CHO-αβ |
Kind gift from Dr Susan Lord, University of North Carolina |
Expressing α- and β-chain of human fibrinogen |
PMID:8418831
|
| Transfected construct(human) |
pMLPAα |
Kind gift from Dr Susan Lord, University of North Carolina |
|
PMID:8418831
|
| Transfected construct(human) |
pMLPAα390 |
This paper |
|
Ariëns Lab |
| Transfected construct(human) |
pMLPAα220 |
This paper |
|
Ariëns Lab |
| Biological sample (human) |
Human plasminogen-depleted plasma-purified fibrinogen |
Merck |
341578 |
|
| Biological sample (human) |
Glu-plasminogen |
Enzyme Research Laboratories |
HPG 2001 |
|
| Biological sample (human) |
FXIII |
Zedira |
T007 |
|
| Biological sample (human) |
Tissue factor(PPP reagent) |
Stago |
86193 |
|
| Biological sample (human) |
Thrombin |
Merck |
605190 |
|
| Antibody |
CD41 Alexa Fluor 700 (anti-mouse monoclonal) (MWReg30) |
BioLegend |
133925 |
(5 µg/mL) |
| Antibody |
CD62P PE (anti-mouse monoclonal) (RMO-1) |
BioLegend |
148305 |
(10 µg/mL) |
| Antibody |
CD63 APC (anti-mouse monoclonal) (NVG-2) |
BioLegend |
143905 |
(5 µg/mL) |
| Antibody |
IgG2a PE (monoclonal Mouse)(MOPC-173) |
BioLegend |
400211 |
(10 µg/mL) |
| Antibody |
IgG2a APC (monoclonal Rat) (RTK 2758) |
BioLegend |
400511 |
(5 µg/mL) |
| Antibody |
FITC fibrinogen (anti-human polyclonal) |
Agilent Technologies |
F0111 |
(60 µg/mL) |
| Peptide, recombinant protein (human) |
WT |
This paper |
|
Purified from CHO cells, WT fibrinogen, Ariëns Lab |
| Peptide, recombinant protein (human) |
α390 |
This paper |
|
Purified from CHO cells, fibrinogen with α-chain truncated at α390, Ariëns Lab |
| Peptide, recombinant protein (human) |
α220 |
This paper |
|
Purified from CHO cells, fibrinogen with α-chain truncated at α220, Ariëns Lab |
| Peptide, recombinant protein (human) |
γ′/γ′ |
This paper |
|
Purified from CHO cells, fibrinogen homozygous in γ′-chain, Ariëns Lab |
| Peptide, recombinant protein (human) |
t-PA |
Pathway Diagnostics |
TC41072 |
|
| Chemical compound, drug |
PAR4 |
Cambridge BioScience |
ANA60218-5 |
|
| Commercial assay or kit |
Alexa Fluor 488 using a protein labelling kit |
Thermo Fisher Scientific |
A10235 |
|
| Commercial assay or kit |
QuikChange II Site-Directed Mutagenesis Kit |
Agilent Technologies |
200524 |
|
| Software, algorithm |
ImageJ software |
ImageJ (http://imagej.nih.gov/ij/) |
RRID:SCR_003070
|
|
| Software, algorithm |
GraphPad Prism |
GraphPad Prism (https://graphpad.com) |
RRID:SCR_015807
|
Version 8 |
| Software, algorithm |
MATLAB |
MathWorks (http://mathorks.com) |
RRID:SCR_001622
|
|
| Software, algorithm |
OriginLab |
OriginLab (http://originlab.com) |
RRID:SCR_014212
|
|
| Other |
Dynabeads M-450 Epoxy |
Invitrogen |
14011 |
|
