Biological sample (Homo sapiens) |
Endometrial biopsies |
University and Polytechnic La Fe Hospital (Valencia, Spain) |
|
Freshly isolated from human donors |
Antibody |
Anti-progesterone receptor antibody [YR85] (rabbit monoclonal anti-human) |
Abcam |
Cat: AB32085RRID:AB_777452
|
Dilution: (1:50) |
Antibody |
Anti-estrogen receptor alpha antibody (mouse monoclonal antibody) |
Santa Cruz |
Cat: sc-8002RRID:AB_627558
|
Dilution: (1:50) |
Antibody |
Goat anti-rabbit IgG H&L (Alexa Fluor 488) (goat polyclonal) |
Abcam |
Cat: ab150077RRID:AB_2630356
|
Dilution: (1:1000) |
Antibody |
Goat anti-mouse IgG (H + L) Cro Alexa Fluor 488 (goat polyclonal) |
Invitrogen |
Cat: A-11001RRID:AB_2534069
|
Dilution: (1:1000) |
Sequence-based reagent |
RT-qPCR primers |
This paper |
|
Supplementary file 3
|
Commercial assay or kit |
QIAsymphony RNA Kit |
Qiagen |
931636 |
Global RNA-seq library preparation |
Commercial assay or kit |
Illumina TruSeq Stranded mRNA sample prep kit |
Illumina |
20020595 |
Global RNA-seq library preparation |
Commercial assay or kit |
Kapa SYBR fast qPCR kit |
Kapa Biosystems Inc |
KK4602 |
Global RNA-seq library preparation |
Commercial assay or kit |
TruSeq RNA CD Index Plate (96 indexes, 96 samples) |
Illumina |
20019792 |
RNA sequencing |
Commercial assay or kit |
NextSeq 500/550 cartridge of 150 cycles |
Illumina |
FC-404-2002 |
RNA sequencing |
Commercial assay or kit |
SuperScript VILO cDNA Synthesis Kit |
Thermo Fisher Scientific |
11754250 |
RT-qPCR. cDNA preparation |
Software, algorithm |
STAR |
Dobin et al., 2013
|
URL: http://code.google.com/p/rna-star/RRID:SCR_004463
|
RNA-seq analysisRead alignerVersion 2.4.2a |
Software, algorithm |
FastQC |
|
URL: http://www.bioinformatics.babraham.ac.uk/projects/fastqc/RRID:SCR_014583
|
RNA-seq analysisQuality of FASTQ file determinationVersion 0.11.2 |
Software, algorithm |
SAMtools |
Li et al., 2009
|
URL: http://htslib.org/RRID:SCR_002105
|
RNA-seq analysisSAM and BAM manipulation files Version 1.1 |
Software, algorithm |
HTSeq |
Anders et al., 2015
|
URL: http://htseq.readthedocs.io/en/release_0.9.1/RRID:SCR_005514
|
RNA-seq analysisTo count the number of reads per geneVersion 0.6.1p1 |
Software, algorithm |
BEDtools |
Quinlan and Hall, 2010
|
URL: https://github.com/arq5x/bedtools2RRID:SCR_006646
|
RNA-seq analysisTo obtain gene coverageVersion 2.17.0 |
Software, algorithm |
edgeR |
Robinson et al., 2010
|
URL: http://bioconductor.org/packages/edgeR/RRID:SCR_012802
|
RNA-seq analysisTo analyze differentially expressed genesVersion 3.24.3 |
Software, algorithm |
String |
Jensen et al., 2009
|
URL: http://string.embl.de/RRID:SCR_005223
|
Interaction Network. |
Software, algorithm |
Cytoscape |
Shannon et al., 2003
|
URL: http://cytoscape.orgSCR_003032 |
Interaction Network |
Software, algorithm |
CytoHubba |
Chin et al., 2014
|
URL: http://apps.cytoscape.org/apps/cytohubbaRRID:SCR_017677
|
Interaction Network |
Other |
Custom scripts |
|
URL: https://github.com/mclemente-igenomix/garrido_et_al_2021
|
The specific script to run RNA-seq analysis |