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. 2021 Oct 28;10:e70753. doi: 10.7554/eLife.70753

Key resources table.

Reagent type (species) or resource Designation Source or reference Identifiers Additional information
Biological sample (Homo sapiens) Endometrial biopsies University and Polytechnic La Fe Hospital (Valencia, Spain) Freshly isolated from human donors
Antibody Anti-progesterone receptor antibody [YR85] (rabbit monoclonal anti-human) Abcam Cat: AB32085RRID:AB_777452 Dilution: (1:50)
Antibody Anti-estrogen receptor alpha antibody (mouse monoclonal antibody) Santa Cruz Cat: sc-8002RRID:AB_627558 Dilution: (1:50)
Antibody Goat anti-rabbit IgG H&L (Alexa Fluor 488) (goat polyclonal) Abcam Cat: ab150077RRID:AB_2630356 Dilution: (1:1000)
Antibody Goat anti-mouse IgG (H + L) Cro Alexa Fluor 488 (goat polyclonal) Invitrogen Cat: A-11001RRID:AB_2534069 Dilution: (1:1000)
Sequence-based reagent RT-qPCR primers This paper Supplementary file 3
Commercial assay or kit QIAsymphony RNA Kit Qiagen 931636 Global RNA-seq library preparation
Commercial assay or kit Illumina TruSeq Stranded mRNA sample prep kit Illumina 20020595 Global RNA-seq library preparation
Commercial assay or kit Kapa SYBR fast qPCR kit Kapa Biosystems Inc KK4602 Global RNA-seq library preparation
Commercial assay or kit TruSeq RNA CD Index Plate (96 indexes, 96 samples) Illumina 20019792 RNA sequencing
Commercial assay or kit NextSeq 500/550 cartridge of 150 cycles Illumina FC-404-2002 RNA sequencing
Commercial assay or kit SuperScript VILO cDNA Synthesis Kit Thermo Fisher Scientific 11754250 RT-qPCR. cDNA preparation
Software, algorithm STAR Dobin et al., 2013 URL: http://code.google.com/p/rna-star/RRID:SCR_004463 RNA-seq analysisRead alignerVersion 2.4.2a
Software, algorithm FastQC URL: http://www.bioinformatics.babraham.ac.uk/projects/fastqc/RRID:SCR_014583 RNA-seq analysisQuality of FASTQ file determinationVersion 0.11.2
Software, algorithm SAMtools Li et al., 2009 URL: http://htslib.org/RRID:SCR_002105 RNA-seq analysisSAM and BAM manipulation files Version 1.1
Software, algorithm HTSeq Anders et al., 2015 URL: http://htseq.readthedocs.io/en/release_0.9.1/RRID:SCR_005514 RNA-seq analysisTo count the number of reads per geneVersion 0.6.1p1
Software, algorithm BEDtools Quinlan and Hall, 2010 URL: https://github.com/arq5x/bedtools2RRID:SCR_006646 RNA-seq analysisTo obtain gene coverageVersion 2.17.0
Software, algorithm edgeR Robinson et al., 2010 URL: http://bioconductor.org/packages/edgeR/RRID:SCR_012802 RNA-seq analysisTo analyze differentially expressed genesVersion 3.24.3
Software, algorithm String Jensen et al., 2009 URL: http://string.embl.de/RRID:SCR_005223 Interaction Network.
Software, algorithm Cytoscape Shannon et al., 2003 URL: http://cytoscape.orgSCR_003032 Interaction Network
Software, algorithm CytoHubba Chin et al., 2014 URL: http://apps.cytoscape.org/apps/cytohubbaRRID:SCR_017677 Interaction Network
Other Custom scripts URL: https://github.com/mclemente-igenomix/garrido_et_al_2021 The specific script to run RNA-seq analysis