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Gel electrophoresis (A), reduced CO-difference spectra (B), and ligand binding spectra (C and D) of the purified L. donovani CYP51. The expected molecular weight of the CYP51-32c construct is 53 kDa. The P450 content was determined to be 7.1 nmol/mg for the batch of purified CYP51 used in this experiment. Various concentrations of 24c (C) and 9h (D) were titrated into CYP51 before recording binding spectra and determining dissociation constants (Kd).
