Figure 1.

Insulin release from isolated pancreatic islets (adapted from Ref. 2). A. Rat pancreatic islets were isolated by the method of Okamoto et al.^2,16) The collagenase treatment was carried out for 10 min (closed circle) or 25 min (open circle). The insulin released from isolated islets was examined as described previously.^16,287) B. Effect of varying periods of collagenase treatment on islet insulin release and on its susceptibility to somatostatin. Results represent the mean ± S.E. in µU of insulin released in 4 incubation flasks. The flask contained 6 isolated islets. +: The insulin release was inhibited by somatostatin. −: The insulin release was not inhibited by somatostatin. ±: Inhibition by somatostatin of insulin release was not prominent.