Table 1.
Nucleation rate and equilibrium constants from previous studies
| Cooper et al. (7) | Frieden (8) | Sept and McCammon (9) | Oda et al. (10) | This study | |
|---|---|---|---|---|---|
| k+1 (μM−1 s−1) | 6.7 × 10−3 | — | 35.7 | — | 3.5 (1.2–11) × 10−6 |
| k−1 (s−1) | 500 | — | 1.63 × 108 | — | 0.041 (0.036–0.047) |
| Kd of dimer (μM) | 75,000 | 800,000 | 4.6 × 106 | 1700 | 12,000 (3300–39,000) |
| k+2 (μM−1 s−1) | 6.7 × 10−3 | — | 2.18 | — | 13 (4.0–40) × 10−5 |
| k−2 (s−1) | 500 | — | 1300 | — | 22 (19–25) |
| Kd of trimer (μM) | 75,000 | 5 | 600 | 5.5 × 105 | 1.7 (0.48–6.3) × 105 |
Two monomers associate reversibly to form a dimer with rate constants of k+1 and k−1. A monomer binds reversibly to a dimer to form a trimer with rate constants of k+2 and k−2. The ratios of the rate constants give the dissociation equilibrium constants, Kd. Bold numbers are assumed values. We report the average of the association rate constants of two data sets reported in Cooper et al. (7) and calculated the dissociation equilibrium constants accordingly. The Sept and McCammon (9) values are also reported in Table 2, column 2. Our values (final column) are the values also reported in Table 2, column 5. Our values are best-fit values followed by 95% confidence intervals in parentheses.