Table 2.
Comparison of rate and equilibrium constants for nucleation reactions
| Sept and McCammon (9) | Local algorithm; defined start value | Multistart fit to polymer only | Multistart fit to polymer and filament ends | Multistart fit to polymer, filament ends, and monomer | |
|---|---|---|---|---|---|
| k+1 (μM−1 s−1) | 35.7 | 9.6 (0.0039–6600) | 4.7 (1.3–32) × 10−6 | 15 (0.96–170) × 10−6 | 3.5 (1.2–11) × 10−6 |
| k−1 (s−1) | 1.63 × 108 | 9.1 (0.016–170,000) × 108 | 0.040 (0.034–0.049) | 0.047 (0.038–0.058) | 0.041 (0.036–0.047) |
| Kd of dimer (μM) | 4.6 × 106 | 9.5 (0.000024–440,000,000) × 107 | 8500 (1100–38,000) | 3100 (220–60,000) | 12,000 (3300–39,000) |
| k+2 (μM−1 s−1) | 2.18 | 0.78 (0.0000015–450) | 9.6 (1.5–35) × 10−5 | 3.4 (0.38–56) × 10−5 | 13 (4.0–40) × 10−5 |
| k−2 (s−1) | 1300 | 15 (12–19) | 27 (23–33) | 21 (18–27) | 22 (19–25) |
| Kd of trimer (μM) | 600 | 19 (0.027–13,000,000) | 2.8 (0.66–22) × 105 | 6.2 (0.32–71) × 105 | 1.7 (0.48–6.3) × 105 |
Best-fit values are followed by 95% CIs in parentheses. The Sept and McCammon (9) values are also reported in Table 1, column 4. The data in column 3 come from fits to the experiments in Fig. 1B. The data in column 4 come from fits to the experiments in Fig. 1E. The data in column 5 come from the fits to experiments in Fig. 2. All fitted parameters in columns 3–6 are practically identifiable.