Table 3.
Comparison of rate and equilibrium constants for nucleation reactions from the standard model with and without reversible severing and with modeling the elongation reactions in two different ways
| Standard | Annealing and severing | Tetramer from two dimers | |
|---|---|---|---|
| k+1 (μM−1 s−1) | 3.5 (1.2–11) × 10−6 | 1.9 (0.79–5.2) × 10−6 | 2.8 (1.4–16) × 10−6 |
| k−1 (s−1) | 0.041 (0.036–0.047) | 0.014 (0.012–0.016) | 0.050 (0.045–0.056) |
| Kd of dimer (μM) | 12,000 (3300–39,000) | 7400 (2300–20,000) | 18,000 (2800–40,000) |
| k+2 (μM−1 s−1) | 1.3 (0.40–4.0) × 10−4 | 55 (3.7–2900) × 10−4 | 0.51 (0.094–0.99) × 10−4 |
| k−2 (s−1) | 22 (19–25) | 5100 (440–9,500,000) | (<0.64) |
| Kd of trimer (μM) | 1.7 (0.48–6.3) × 105 | 930,000 (1500–26,000,000,000) | (<68,000) |
| k+S (s−1) | — | 2.5 (2.3–2.7) × 10−4 | — |
| k−S (μM−1 s−1) | — | 2.4 (2.0–2.8) | — |
| K of annealing (μM) | — | 9600 (7400–12,000) | — |
| k+3 (μM−1 s−1) | — | — | 0.23 (0.0071–1.2) |
| k−3 (s−1) | — | — | (<5.0) |
| Kd of tetramer to dimer (μM) | — | — | (<700) |
Best-fit values are followed by 95% CIs in parentheses. For parameters that are not identifiable, we did not include the best-fit value for they were unrealistically small (1.1 × 1015 for k−2, 2.2 × 10−11 for Kd of trimer, 4.7 × 10−17 for k−3, and 4.7 × 10−17 for Kd of tetramer to dimer) but do include their upper boundary in parentheses.