Table 2.
The secretome of MSCs from Wharton jelly (WJ), perivascular region (PRV) and umbilical cord membrane (UCM). Marked concentrations represent the statistically significant differences (*p ≤ 0,05) v. passage 3. The rest of analyzed proteins concentrations remained unchanged without significant differences. The results of remaining analytes (FGF-2, GM-CSF, IFNγ, IL-10, IL-12p40, IL-17A, IL-2, IL-3, IL-4, IL-5, IL-7, MIP-1α, MIP-1β, TGF-β3) were at a very low level or outside the method resolution and were not used in this analysis
| WJ | PRV | UCM | ||||
|---|---|---|---|---|---|---|
| protein/ | P3 | P8 | P3 | P8 | P3 | P8 |
| (pg/ml) | ||||||
| IL-6 | 1070.1 ± 177.1 | 911.0 ± 225.2 | 539.8 ± 82.5 | 1900.3 ± 1439.1 | 612.3 ± 189.0 | 1690.3 ± 592.0* |
| TGF-β1 | 535.6 ± 194.7 | 398.0 ± 65.0 | 809.7 ± 151.4 | 606.5 ± 153.7 | 529.6 ± 172.4 | 607.5 ± 151 |
| IL-8 | 367.0 ± 44.1 | 785.7 ± 170.1* | 134.0 ± 25.9 | 415.3 ± 206.6* | 211.9 ± 144.7 | 309.6 ± 266.1 |
| MCP-1 | 121.8 ± 58.4 | 73.7 ± 11.4 | 93.9 ± 19.5 | 71.0 ± 7.8 | 505.9 ± 130.6 | 614.5 ± 364.4 |
| TGF-β2 | 68.9 ± 20.8 | 165.8 ± 47.3* | 139.2 ± 77.0 | 144.1 ± 19.8 | 67.2 ± 15.6 | 270.1 ± 17.6* |
| IP-10 | 16.6 ± 1.3 | 26.5 ± 3.7* | 6.8 ± 2.0 | 24.6 ± 4.2* | 14.3 ± 3.2 | 38.1 ± 12.0* |
| G-CSF | 14.9 ± 5.3 | 10.8 ± 3.6 | 29.4 ± 4.8 | 14.6 ± 5.6 | 13.9 ± 5.1 | 945.9 ± 596* |
| PDGF AA | 11.1 ± 2.6 | 35.6 ± 7.3* | 56.6 ± 4.7 | 8.3 ± 3.1* | 7.7 ± 1.9 | 15.6 ± 13.2 |
| Fractalkine | 27.2 ± 5.3 | 35.8 ± 5 | 22.0 ± 7.4 | 35.1 ± 4.9 | 20.5 ± 10.8 | 36.2 ± 14.3 |
| IL-13 | 3.7 ± 1.1 | 3.4 ± 0.4 | 3.8 ± 1.0 | 3.3 ± 0.3 | 2.7 ± 0.1 | 3.9 ± 1.0 |
| IFNα | 8.4 ± 1.5 | 12.4 ± 2.3 | 9.4 ± 0.8 | 11.1 ± 3.4 | 10.3 ± 1.0 | 13.3 ± 5.6 |
| Flt-3 L | 5.6 ± 2.7 | 5.9 ± 2.2 | 4.8 ± 1.9 | 5.7 ± 3.4 | 5.8 ± 2.7 | 9.7 ± 4.0 |
| EGF | 4.4 ± 0.6 | 4.8 ± 0.4 | 4.2 ± 0.6 | 5.0 ± 0.5 | 4.0 ± 0.0 | 5.9 ± 1.0 |
| TNFα | 1.7 ± 0.3 | 1.4 ± 0.0 | 1.6 ± 0.0 | 1.6 ± 0.1 | 1.4 ± 0.3 | 1.8 ± 0.4 |
| IL-1β | 1.3 ± 0.1 | 1.3 ± 1.0 | 0.8 ± 0.2 | 1.1 ± 0.9 | 1.2 ± 0.0 | 3.5 ± 2.9 |