Fig. 4.

The cultivated tomato accumulated more secondary metabolites than the wild species [obtained by ESI(+) mode]. Samples used for the RNASeq analysis and the qPCR verification were used for determination of secondary metabolites by LC–FTICR–MS and LC–ESI(+)–MS/MS. a Mono-caffeoylquinic acid (CQA) (3 regioisomers, I–III), and b caffeoylglucaric acid (4 regioisomers, I–IV), Quer-3-O-Hex-Pent-DHex, Kaemp-3-O-Hex-DHex (2 regioisomers, I and II), rutin and Quer-3-O-Hex-DHex I were unambiguously identified. Quantification of them was performed with LC–MS/MS by use of their corresponding MRM transitions. Peak area ratios (above) and their normalized illustration (below) are shown as mean of two independent biological experiments