Table 1.
Gene | Sequence forward | Sequence reverse |
---|---|---|
CHS | AGCTTAAGGAGAAATTTAAGCGCA | AGCATCCAAAGAAGGAGCCA |
CHI | TCACCGCTTGGATCATTGACA | CACTTTGCTGCAGGGGAAAC |
F3H | GGATCACTGTTCAGCCCGTT | GCATTCTTGAACCTTCCATTGCT |
FLS | TTTGGCCTCCTCCTGCTATT | CATGGCCTTCCAAACCAAGC |
F3’H | GGGCGAAACACATGGCTTAT | TAAGGCGCGTGTAAGTGTTC |
F3’5’H | TGCTTCTACCCCTAATGCTGC | CCAACGTGGTCCATAGGGTG |
DFR | GATGGTGCGACAGAAATGGC | AAGACACTACGGGCAAGTCC |
LDOX | GTGGTCAGCTTGAGTGGGAG | TAGGTTCCTGATCTGCTTGGC |
PP2A | CGATGTGTGATCTCCTATGGTC | AAGCTGATGGGCTCTAGAAATC |
Ubi10 | GGACGGACGTACTCTAGCTGAT | AGCTTTCGACCTCAAGGGTA |
Act | GAAATAGCATAAGATGGCAGACG | ATACCCACCATCACACCAGTAT |
Primer pairs were used with both species. The sequences are given in direction.
CHS chalcone synthetase, CHI chalcone isomerase, F3H flavanone-3-hydroxylase, FLS flavonol synthase, flavonoid-3-hydroxylase, flavonoid--hydroxylase, DFR dihydroflavonol-4-reductase, LDOX leucoanthocyanidin dioxygenase, PP2A proteinphosphatase 2, Ubi10 ubiquitin 10, Act actin