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. 2021 Oct 22;107(3):177–206. doi: 10.1007/s11103-021-01194-0

Table 1.

Used oligonucleotide primer pairs

Gene Sequence forward Sequence reverse
CHS AGCTTAAGGAGAAATTTAAGCGCA AGCATCCAAAGAAGGAGCCA
CHI TCACCGCTTGGATCATTGACA CACTTTGCTGCAGGGGAAAC
F3H GGATCACTGTTCAGCCCGTT GCATTCTTGAACCTTCCATTGCT
FLS TTTGGCCTCCTCCTGCTATT CATGGCCTTCCAAACCAAGC
F3’H GGGCGAAACACATGGCTTAT TAAGGCGCGTGTAAGTGTTC
F3’5’H TGCTTCTACCCCTAATGCTGC CCAACGTGGTCCATAGGGTG
DFR GATGGTGCGACAGAAATGGC AAGACACTACGGGCAAGTCC
LDOX GTGGTCAGCTTGAGTGGGAG TAGGTTCCTGATCTGCTTGGC
PP2A CGATGTGTGATCTCCTATGGTC AAGCTGATGGGCTCTAGAAATC
Ubi10 GGACGGACGTACTCTAGCTGAT AGCTTTCGACCTCAAGGGTA
Act GAAATAGCATAAGATGGCAGACG ATACCCACCATCACACCAGTAT

Primer pairs were used with both species. The sequences are given in 5-3 direction.

CHS chalcone synthetase, CHI chalcone isomerase, F3H flavanone-3-hydroxylase, FLS flavonol synthase, F3H flavonoid-3-hydroxylase, F35H flavonoid-3,5-hydroxylase, DFR dihydroflavonol-4-reductase, LDOX leucoanthocyanidin dioxygenase, PP2A proteinphosphatase 2, Ubi10 ubiquitin 10, Act actin