Table 2.
Mendelian ratios of FHL1 and/or FHL2 global knockout crossings
| FHL1. FHL1 global knockout (Sheikh et al. 2008). Breeding of wildtype males with heterozygous FHL1 females. Please note, FHL1 is X-linked. | |||
| Genotype | Wildtype (both males and females) | Heterozygous (females only) | Hemizygous (males only) |
| Expected ratios | 50% | 25% | 25% |
| Number of animals and percent of genotyped animals at weaning stage (P21) | 91 (55.5%) | 35 (21.3%) | 38 (23.2%) |
| FHL2. FHL2 global knockout (Chu et al. 2000a). Crossing of heterozygous males and females. | |||
| Genotype | Wildtype | Heterozygous | Homozygous |
| Expected ratios | 25% | 50% | 25% |
| Number of animals and percent of genotyped animals at weaning stage (P21) | 26 (22.6%) | 60 (52.2%) | 29 (25.2%) |
| FHL1/2 double knockouts. Crossing of FHL1 (y/+); FHL2 (−/−) males with FHL1 (+/−); FHL2 (−/−) females. Please note that the breeding was performed using a FHL2 homozygous knockout background that showed no changes to viability or fertility (Chu et al. 2000a). | |||
| Genotype | FHL2 knockout, FHL1 Wildtype (both males and females) | FHL2 knockout , FHL1 Heterozygous (females only) | FHL2 knockout , FHL1 Hemizygous (males only) |
| Expected ratios | 50% | 25% | 25% |
| Number of animals and percent of genotyped animals at weaning stage (P21) | 29 (69.1%) | 9 (21.4%) | 4 (9.5%) |