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. 2021 Oct 28;12:6215. doi: 10.1038/s41467-021-26524-0

Table 1.

Michaelis–Menten parameters from lysate kinetic data.

Strain StlA variant Vmax (μmol TCA/min) KM (mM Phe)
EP2315 wild-type 3.20 ± 0.63 1.69 + 0.37
EP2516 S92G_H133F_A433S_V470A 11.96 ± 1.64 4.06 + 0.44
EP2525 S92G_H133M_I167K_L432I_V470A 10.70 ± 1.89 4.40 + 0.27

Vmax and KM average of n = 3 biological replicates ±s.d.