Table 1.

Summary of the analysis of the 35 metabolism genes in human M0, M1-like, and M2-like macrophages upon interacting with PDA tumor cells

ALDH1a3-related genes (genes in the glucose metabolism pathway)			ALDH3A1	FAHD1	GSTP1	ACADM	HK3
	M0 vs. M0.CO	DNA methylation Array	- A	–	M	–	M
		RNA sequence	/B	↓	↓	↓	↓
	M1 vs. M1.CO	DNA methylation Array	MC	–	M	M	M
		RNA sequence	↓D	↓	↓	↑	↓
	M2 vs. M2.CO	DNA methylation Array	–	–	–	–	M
		RNA sequence	/	↑E	↓	↓	↓
			HK1	PFKP	ALDOA	GAPDHS	PGK1
	M0 vs. M0.CO	DNA methylation Array	M	–	M	–	–
		RNA sequence	↓	↑	↓	/	↓
	M1 vs. M1.CO	DNA methylation Array	M	M	M	M	–
		RNA sequence	↑	↑	↑	/	↓
	M2 vs. M2.CO	DNA methylation Array	M	–	M	–	–
		RNA sequence	↑	↓	↑	/	↑
			PGAM1	LDHC	LDHB
	M0 vs. M0.CO	DNA methylation Array	–	–	M
		RNA Sequence	↓	/	↓
	M1 vs. M1.CO	DNA methylation Array	M	M	M
		RNA sequence	↑	↓	↓
	M2 vs. M2.CO	DNA methylation Array	–	–	M
		RNA sequence	↑	/	↓
ALDH1a3-related genes (genes in other metabolism pathways)			TPO	GSTM4	GSTK1	GSTA4	UGT2A3
	M0 vs. M0.CO	DNA methylation Array	–	–	–	M	–
		RNA sequence	/	↓	↓	/	/
	M1 vs. M1.CO	DNA methylation Array	M	M	M	M	–
		RNA sequence	/	↓	↓	/	/
	M2 vs. M2.CO	DNA methylation Array	–	–	–	–	–
		RNA sequence	/	↓	–	/	↑
			CYP3A43	ABAT
	M0 vs. M0.CO	DNA methylation Array	–	–
		RNA sequence	/	/
	M1 vs. M1.CO	DNA methylation Array	–	M
		RNA sequence	/	↑
	M2 vs. M2.CO	DNA methylation Array	–	–
		RNA sequence	/	↓
NQO-1 related genes (genes in the OXPHOS pathways)			NDUFS6	NDUFA10	NDUFA12	NDUFB9	UQCRQ
	M0 vs. M0.CO	DNA methylation Array	–	–	–	–	–
		RNA sequence	↓	↓	↓	↓	↓
	M1 vs. M1.CO	DNA methylation Array	M	M	M	M	–
		RNA sequence	↓	↓	↓	↓	↓
	M2 vs. M2.CO	DNA methylation Array	–	–	–	–	–
		RNA sequence	↑	↑	↓	↑	↑
			COX7A1	COX7B2	ATP5D	ATP5G2	ATP6V1A
	M0 vs. M0.CO	DNA methylation Array	–	–	M	M	–
		RNA sequence	↑	/	↑	↓	↓
	M1 vs. M1.CO	DNA methylation Array	M	M	M	M	M
		RNA sequence	↓	/	↑	↑	↓
	M2 vs. M2.CO	DNA methylation Array	–	–	M	M	–
		RNA sequence	↓	/	↑	↓	↓
			ATP6V1E1	ATP6V0D1	ATP6V0D2	ATP6V0E1	ATP4A
	M0 vs. M0.CO	DNA methylation Array	–	M	–	–	–
		RNA sequence	↓	↓	↓	↓	/
	M1 vs. M1.CO	DNA methylation Array	M	M	M	M	M
		RNA sequence	↑	↑	↓	↓	/
	M2 vs. M2.CO	DNA methylation Array	–	–	–	–	M
		RNA sequence	↓	↓	↓	↓	/

^A-: no significant change, ^B/: not performed, ^CM: methylation; ↑; ^D ↓ : downregulated; ^E ↑ : upregulated