Fig. 3. elavl2 alternative splicing is dynamic during development.

a Multiple products are detected from RT-PCR of RNA from larval brain in the alternatively spliced part of bee elavl2 on an agarose gel. M: Marker b, c Top and bottom part of a representative 5% denaturing polyacrylamide gel separating ³²P-labeled alternative splice products from larval brains. Length of PCR products from splice variants are indicated at the right in bold for prominent products and in italics for very rare products. Vertical lines at the right of indicated splice variants indicate inseparable products. M: Marker. d, e Analysis of alternative splicing from larval brains proximal (KpnI) and distal (FokI) of exon 4 from ³²P-labeled labeled forward (d) or return (e) primer after digestion with either KpnI or FokI on a representative 5% denaturing polyacrylamide gels. Length of PCR products from splice variants are indicated at the right in bold for prominent products and in italics for very rare products. M: Marker. f–h Developmental and sex-specific alternative splicing of bee elavl2 quantified from denaturing polyacrylamide gels shown as mean with the standard error from three independent replicates as percent from all splice products from top (f left) and bottom (f right) gel parts and after KpnI or FokI digestion as above from embryos (yellow), larval brains (green), drone brains (light blue), worker brains (dark blue) and queen brains (black). (f and g). ^#The 120 nt product can be either 3, 4c, 5 or 3, 3a, 4d, 5. (h) ^The 123 nt products are either 4b, 4c, 4d, 5 or 3, 4c, 4d, 5. *The 120 nt products are either 4b, 4c, 5 or 3, 4c, 5 or 3, 3a, 4d, 5. ⁺The 80 nt products are either 4b, 5 or 3, 5 +/− 4d. The source data underlying this figure are available in Supplementary Data 1.