Fig. 6. elavl2 alternative splicing and expression levels change upon learning.

a Learning response of bees grouped into fast and slow learners (fast learners n = 10 and slow learners n = 8). b elavl2 mRNA expression levels determined by qPCR 0, 2, and 4 h after training shown as mean with the standard error from two to four independent replicates central brains normalized to Appl expression (p = 0.13). c Inclusion levels of elavl2 exons 3a and 4c quantified from RNA in situ hybridizations from control (unpaired) and fast learning (paired) bees in the mushroom body and the medulla (p < 0.05, n = 7). d, e Representative RNA in situ hybridizations against exon 3c in mushroom bodies of control (d) and fast learning (e) bees 2 h after training. Scale bars are 30 µm in E and 6 µm in the inset. The source data underlying this figure are available in Supplementary Data 1.