Fig. 6. High Itgβ8 score on T-cell infiltrating melanoma correlates with patients' worse prognostic and CD8 T-cell activation in the TME.
Transcriptome signatures of Itgβ8pos Treg cells from patient melanoma were extracted from single-cell RNA-seq data (as described in “Methods”). The extracted signatures were used for testing their association with overall survival on The Cancer Genome Atlas (TCGA) RNA-seq data from melanoma. Tumor samples were classified into low and high, based on the expression on ITGB8 score. a Graph illustrates the overall survival of cancer patients stratified by ITGβ8 score in tumor-infiltrating T cells with time expressed in days. n = 358 patients, 179 patients with high expression, 179 patients with low expression log-rank P = 0.0051 using Mantel Cox test. b Graph illustrating the inverse correlation between with ITGβ8 Tregs core and the activated CD8 T-cell score in the same melanoma. Each dot represents one tumor. Spearman R value of correlation: −0.67.