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. 2021 Oct 28;11:21262. doi: 10.1038/s41598-021-00581-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Coefficients of the 44 relationships for 1st- and 2nd-degree relatives from the Neolithic site of Koszyce¹⁵. Each vertical set of 4 coloured points corresponds to one pair of individuals tested with different numbers of aligned reads, according to the legend. Relationships to the left of the solid vertical line are known to be 1st-degree, as per Schroeder et al.¹⁵, and relationships to the right of the line are expected to be 2nd-degree. Grey areas define 1st- and 2nd-degree range intervals.