FIG. 2.

Effects of TLC1 mutations on replicative potential. For each of the tlc1 mutant alleles, residues 476 to 474 are shown; mutated residues are in lowercase and highlighted in gray. Colony growth of strains bearing the indicated tlc1 allele was ranked by approximate colony diameter from + (very small irregularly shaped colonies) to ++++ (indistinguishable from wild type). Colony phenotypes were assessed following one through five restreaks after the loss of a plasmid containing the TLC1 gene.