FIG. 4.

Effects of TLC1 mutations on telomere length. (A and C) Southern analysis of XhoI-digested genomic DNA hybridized to a wild-type telomeric repeat oligonucleotide. Genomic DNA was isolated from strains after 35, 45, 55, and 65 (A) or 25 (C) generations of growth in the presence of the indicated TLC1 allele. Filled arrowheads indicate non-Y′-containing telomeres; the open arrowhead and brackets indicate Y′-containing telomeres. (B) DNA sequences of one strand of the synthetic duplex DNA oligonucleotides used in the gel shift experiment shown in Fig. 3, aligned with the same strand of the tlc1-476gCA mutant gel shift probe. Sequence of two telomeric repeats subcloned from tlc1-476gCA telomeres are shown aligned below.