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. 2021 Oct 15;12:733805. doi: 10.3389/fphar.2021.733805

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Copyright © 2021 Shi, Tang, Ai, Zeng, Yang, Zhu, Wei, Feng, Wen, Pu and He.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SchB ameliorates THP-induced abnormal expression of apoptosis-related proteins in rat heart. (A) Screening of optimum treatment concentration of SchB. (B) Observation on the effect of coculture of SchB and CsA. (C) ROS staining in each group. (D) Quantification of relative fluorescence intensity of ROS in each group. (E) H9C2 cells in CON, SchB, and CsA groups showed bright green fluorescence, while only weak green fluorescence was observed in the THP group. The green fluorescence was more obvious in SchB + THP and CsA + THP groups, which was greatly improved compared with THP. Ionomycin was a strong positive control. Nonquenched Cyt Flu was that the cytoplasmic fluorescence is not quenched. (F) Fluorescence quantitative analysis. (G) Mitochondrial swelling was analyzed for 10 min. All values are mean ± SD. ^* p < 0.05 vs. CON, ^** p < 0.01 vs. CON, ^# p < 0.05 vs. THP, and ^## p < 0.01 vs THP.