FIGURE 1.

Experimental setup, computer program, and data output (A) Experimental setup for dFRAME method. A group of 18 flies were monitored individually and the coordinate data are used for analysis (B) Operational flowchart of the “FlyFeeding” program. The right part represents a screenshot of the program start; the cut-off thresholds can be assigned by users (red circles). The two numbers in red rectangles indicate the analyzing period (3,600 s) and fly body length (11 mm) (C) “FlyFeeding” simultaneously analyzes a group of 18 flies, generating a set of six graphs and three data flies. Graphs i–iv show the analytical results of a representative fly, including the movement tracks (i), the FAEn distribution according to either the residence time (ii) or in every hour of the day (iii), and the FAErt distribution in every hour of the day (iv). The averaged distribution of FAEn and FAErt of the 18 flies are shown in every hour of the day (v and vi, respectively). In addition, the program generates three data files, “Activity.csv” for FAEn every hour, “Feeding.csv” for total FAErt every hour, and “FeedingPDF.csv” for FAErt of each time. All data are listed for individual flies (D) Schematic diagram of food-approaching events (FAE). The brown line indicates the food surface. The gray area represents a fly body length from the food surface, which is determined as feeding zone. A short period of movement track was clipped out, and those close to the food end are shown in blue lines. Once the fly enters the zone, the FAEn counts 1, and the duration that the fly stays in the zone is recorded as the FAErt.