Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Oct 15;15:647117. doi: 10.3389/fnins.2021.647117

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Tomita, Ban, Kato and Kume.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

The sleep-promoting PB interneurons form synaptic contacts with the wake-promoting PFN neurons. (A) The dendritic arbors and presynaptic terminals of the PFN neurons in R52B10-Gal4 were visualized by expression of the postsynaptic marker DenMark and the presynaptic marker syt-GFP, respectively. (B) The neuronal connection of the sleep-promoting PB interneurons to the wake-promoting PFN neurons was revealed using the t-GRASP method. The pre-t-GRASP and the post-t-GRASP encode the split-GFP fragments, which are targeted to presynaptic endings and dendritic terminals, respectively. Maximum-intensity projection of the confocal images of the brain expressing pre-t-GRASP with R59E08-Gal4 and post-t-GRASP with R52B10-LexA. Scale bars represent 100 μm.